【综述】湖南大学聂舟教授:核酸纳米技术与CRISPR-Cas系统结合的进展


本期推荐的是湖南大学聂舟教授团发表在Journal of Analysis and Testing 2021年第2期的综述文章“核酸纳米技术与CRISPR-Cas系统结合的进展”。敬请阅读。


CRISPR-Cas系统与核酸纳米技术结合的进展

The microbial adaptive immune systems composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas), have been repurposed as revolutionary tool kits in many fields, including gene editing, transcriptional regulation, bioimaging and biosensing. Owing to the unprecedented programmability of base paring in nucleic acids, the progress in nucleic acid nanotechnology has brought new inspiration to CRISPR-Cas system. In this mini review, we summarized the research progress of the integration of nucleic acid nanotechnology into CRISPR-Cas system, including delivery of Cas proteins by DNA nanovehicles, conditional CRISPR-Cas system based on dynamic RNA nanotechnology, coupling of CRISPR-Cas and DNA origami. We also discussed the development prospects of the potential combinations of CRISPR-Cas system and nucleic acid nanotechnology.



Fig.1 Classification of CRISPR-Cas systems. a Architecture of class1 and class 2 CRISPR-Cas systems. b Modules of the CRISPR-Cas systems (Types I–VI).

Fig.2 a Design of the CRISPR interference system.  b CRISPR imaging system. By designing different sgRNAs, CRISPR imaging of human telomeres in RPE cells can be performed. c Fluorescent labeled sgRNA realizes intracellular nucleic acid imaging.

Fig.3 a Principle of DETECTR and its potential application fields. b Single-molecule nucleic acid detection based on SHERLOCK. c Schematic of the four-channel multiplexing nucleic acid detection in SHERLOCKv2.
Fig.4 a DNA origami shapes. b 3D meshes rendered in DNA. c The structure of DNA bricks is similar to that of LEGO? bricks.

Fig.5 a  Schematic of DNA NCs design based on CRISPR-Cas9 delivery system. b Schematic of the TDNs-EV design and target delivery.

Fig.6 a The structural design of PAM antenna on DNA origami and the atomic force microscopy (AFM) characterization of one-layered PAM antenna. b Schematic of the effect of Cas12a activities on DNA origami structure. c Schematic of the release of small molecules, enzymes, NPs and living cells from the Cas12a-mediated hydrogel.

Fig.7 a Constitutively active terminator switch cgRNAs (ON?→?OFF logic) with silencing dCas9 in bacteria. b Constitutively inactive toehold switch cgRNAs (OFF?→?ON logic) with silencing dCas9 in bacteria. c Conditional CRISPR-Cas function. d Logic gate implementation using RNA triggers on cgRNA designs.


Fig.8 a Schematic of CRISPR-Cas12a biosensor regulated by fDNAs. b Schematic of pathogen detection based on CRISPR-Cas13a system. c Schematic of the amplification and detection of miRNA using CRISPR-CHA method. d Rational design of CONAN.

本文引文信息

Wang, K., Xu, BF., Lei, CY. et al. Advances in the Integration of Nucleic Acid Nanotechnology into CRISPR-Cas System. J. Anal. Test. 5, 130–141 (2021). https://doi.org/10.1007/s41664-021-00180-1

聂舟教授简介

聂舟湖南大学二级教授,博士生导师。现任湖南大学研究生院副院长兼学位办主任,生物大分子化学生物学湖南省重点实验室主任,第十二届湖南省政协委员。入选国内高层次人才计划,荣获中国化学会青年化学奖、卢嘉锡优秀导师奖等奖励。主持国家自然科学基金杰出青年基金、重点项目、优秀青年基金和国家重点研发计划项目课题等国家级科研项目。围绕生物医学中关键机制,如表观遗传及细胞信号传导等开展深入探索,主要开展以下两方面研究:1. 基于功能蛋白质和核酸的生物传感新方法;2. 化学手段调控细胞行为和命运。自2009年以来作为通讯作者在J. Am. Chem. Soc., Angew. Chem. Int. Ed., Science Advances, Nano Lett., ACS. Nano., Nucleic Acids Res., Chem. Sci., Anal. Chem. 等国际国内高水平期刊上发表学术论文90多篇,近五年论文他引2000多次。获国家发明专利授权2项,受邀为Springer出版社撰写英文学术专著2章。

扫描或长按下方二维码,直达原文页面,阅读全文

点击“阅读原文”,直达本论文原文页面。

##########
<del id='YcBJwiVl'><kbd></kbd></del><tt id='RMm'><bdo></bdo></tt>
<sup id='FpxFQ'><basefont></basefont></sup>
<b id='WVBP'><var></var></b><dfn id='rohJOsT'><address></address></dfn>
<font id='HKBMpjQ'><font></font></font><center id='AJG'><person></person></center>
<acronym id='tmO'><listing></listing></acronym>
    <optgroup id='rWwhS'><small></small></optgroup><small id='itREr'><span></span></small><optgroup id='EElhsZc'><font></font></optgroup><kbd id='wewv'><label></label></kbd>